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KMID : 1005220150300040271
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Journal of Embryo Transfer
2015 Volume.30 No. 4 p.271 ~ p.276
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Motility, Fertilizability and Subsequent Embryonic Development of Frozen-thawed Spermatozoa derived from Epididymis in Hanwoo
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Yang Byoung-Chul
Kang Sung-Sik
Park Chang-Seok
Kim Ui-Hyung
Kim Hyeong-Cheol
Jeon Gi-Jun
Kim Si-Dong
Lee Seok-Dong
Lee Hyun-Jae
Cho Sang-Rae
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Abstract
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The aim of the study was to investigate the ability of sperm derived from the epididymis in regard to sperm motility, sperm penetration to oocyte and subsequent development of the embryo. Frozen-thawed sperm from epididymis showed similar percentage of motile sperm (VSL ≥ 25 μm/sec) as compared to that of commercial sperm (control). Sperm penetration of frozen-thawed epididymal and commercial sperm was not significantly different. Moreover, cleavage and blastocyst rates were similar in both epididymal and control. Sperm derived from the epididymis also showed fertilizability and subsequent embryonic development
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KEYWORD
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epididymis , Hanwoo , sperm
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